FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309894
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG656320.49310685719961406No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTACGG411360.3090633178596313No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGGG294890.22155698610372104No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGTG264180.19848392481562963No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGAGTAGGG239970.180294448625962Illumina Single End PCR Primer 1 (95% over 24bp)
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGGG228930.17199986716648533No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTACGG213340.16028677613811199No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTACGG196330.14750680959592916No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT155850.11709334424451463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)