FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13084474
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCACACGTCTGAACT715640.5469383026019999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCACACGTCTG546980.41803743887602973Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTTCTAACAATAAAGCGTGCTAATGAGAGATCGGAAGTGCACACGTCTG241490.18456225294192186Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACACGTCTGAA228280.17446631786650346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGCCTATCTTTGCTCTGCCACTACAGATCGGAAGAGCACACGTCTGAA226380.17301421516829793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACACGTCTGAA181600.1387904473653278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGAACGGAAGTGCACACGTCTG170660.130429392881976No Hit
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCAAACGTCTG143480.10965668165185699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)