FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12234123
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCACACGTCTGAACT1053930.8614675526803188Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACACGTCTGAA756680.6184995851357715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCACACGTCTG674280.5511469845447852Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACACGTCTGAA396980.32448586629380793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCCGAAGAGCACACGTCTGAA269860.22057976693548037Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACAAGTCTGAA266160.21755543899632201Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CACCTCCACACATCTACGACCAACGTAGATCGGAAGAGCACACGTCTGAA247870.20260545034572566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCAGAGGTCTGAACT226730.18532591179604782No Hit
GAAAGGTGTAGGACCCGCTCGTAGACCGAGAACGGAAGTGCACACGTCTG214150.17504319680290936No Hit
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCAGACGTCTGAACT197210.16119667915714106Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACAAGTCTGAA195830.16006868657442794Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCAAACGTCTG190920.15605532166057182No Hit
GGTGATAGGGAAGACATTCACTAAAGAGATCGGAAGAGCACACGTCTGAA187370.1531536016108388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCCGAAGAGCACAAGTCTGAA170150.13907821590480987No Hit
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACACGTCTGCA155620.127201598349142Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCCGAAGAGCACACGTCTGAA153770.1256894343795628Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCACAGGTCTGAACT152890.1249701347616008Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCAGACGTCTGAA131030.10710207834268137Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCCGAAGAGCACAAGTCTGAA126430.10334210306697098No Hit
CGTTGCGTAACACGTCTGACGGCTTAGAAGATCGGAAGTGCACACGTCTG122700.10029325354992753Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)