FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12234123
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGACTAGGG553910.45275824021059785No Hit
CCAGTAGCGTGAATGGCTCCCACAAGATCGGAAGAGCGTCGTCTCGGGAA283840.232006822229922No Hit
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGACTACGG202180.1652590872267673No Hit
CGACACCTATAGGCCTGAGCAGCAATAGATCGGAAGAGCGTCGACTAGGG186410.15236891111851664No Hit
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGAGTAGGG165250.13507302485024877Illumina Single End PCR Primer 1 (95% over 24bp)
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGACTAGTG150800.12326179816894109No Hit
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCCGAAGAGCGTCGACTAGGG146520.1197633863906714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)