FastQCFastQC Report
Thu 31 Aug 2023
EGAF00007985517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236886
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCACACGTCTGAACT4029972.094918065221159Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACGGACTGCCTATACTGGGTACGAAAGATCGGAAGAGCACACGTCTGAA2242271.1656096522067032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCAGACGTCTGAACT1695320.8812860875715539Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CACGGACTGCCTATACTGGGTACGAAAGATCGGAAGAGCACAAGTCTGAA972120.5053416649659409Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CACGGACTGCCTATACTGGGTACGAAAGATCCGAAGAGCACACGTCTGAA904710.47029961086217387Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTACTGAATTTCAGAGTAGTCAATTCGAGATCGGAAGTGCACACGTCGG855870.4449108863045713No Hit
CACGGACTGCCTATACTGGGTACGAAAGATCCGAAGAGCACAAGTCTGAA719550.3740470261143098No Hit
CACGGACTGCCTATACTGGGTACGAAAGATCGGAAGAGCACACGTCTGCA569140.29585869563296263Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTACTGAATTTCAGAGTAGTCAATTCGAGATCGGAAGTGCAAACGTCGG547450.28458348196272515No Hit
TGTGGGAGCCATTCACGCTACTGGAGATAGGAAGAGCACACGTCTGAACT516080.2682762688306205Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACTCGTTTCTACTACTCCTAGGTCAGATCGGAAGAGCACACGTCTGAACT393500.20455493680214148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTACTGAATTTCAGAGTAGTCAATTCGAGAACGGAAGTGCACACGTCGG361920.188138558392455No Hit
GTACGTACTTAAGCTACGGCCGACTTACTAAGATCGGATGTGAACACGTC356760.1854562115718729No Hit
CACGGACTGCCTATACTGGGTACGAAAGATCGGAAGAGCAGACGTCTGAA307520.1598595531522098Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTTACTGAATTTCAGAGTAGTCAATTCGAGAACGGAAGTGCAAACGTCGG298880.15536818173170025No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA295990.1538658595783122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACGGACTGCCTATACTGGGTACGAAAGATCGGAAGAGCACAAGTCTGCA285250.1482828353819844Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TATCTACATTCGCCGTTGTTACTAACAGAGATCGGAAGTGCACACGTCGG278340.14469077791488705No Hit
GTACGTACTTAAGCTACGGCCGACTTACTAAGATCGGATGTGAACACGGC269580.14013702633575933No Hit
AGAGGTTATCGGACTACCGCAGCGTGCGAAAGATCGGATGTGAACACGTC261590.1359835474410983No Hit
CACGGACTGCCTATACTGGGTACGAAAGATCCGAAGAGCACACGTCTGCA257960.13409654764289813Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACGGACTGCCTATACTGGGTACGAAAGATCCGAAGAGCACAAGTCTGCA236500.12294089594334552No Hit
GTTACTGAATTTCAGAGTAGTCAATTCGAGATCGGAAGTGCACACGTCTG215230.11188401282827168Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TATCTACATTCGCCGTTGTTACTAACAGAGATCGGAAGTGCAAACGTCGG212130.11027252539730183No Hit
GTGCGCATGCATCCATTCCGTTCGAGATCGGAAGAGCACACGTCTGAACT210130.1092328560869987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)