FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236886
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTACCCAGTATAGGCAGTCCGTGAGATCGGAAGAGCGTCGACTAGGG1249860.6497205420877371No Hit
CCAGTAGCGTGAATGGCTCCCACAAGATCGGAAGAGCGTCGTCTCGGGAA1061570.5518408748692486No Hit
TTCGTACCCAGTATAGGCAGTCCGTGAGATCGGAAGAGCGTCGACTACGG590240.30682720685666065No Hit
CCAGTAGCGTGAATGGCTCCCACAAGATCGGAAGAGCTTCGTCTCGGGAA448460.2331250494492716No Hit
CCAGTAGCGTGAATGGCTCCCACAAGATCGGAAGAGCGTCGTCTAGGGAA422430.2195937533756763Illumina Single End PCR Primer 1 (96% over 26bp)
TTCGTACCCAGTATAGGCAGTCCGTGAGATCGGAAGAGCGTCGACTAGTG416150.21632919174132448No Hit
TTCGTACCCAGTATAGGCAGTCCGTGAGATCCGAAGAGCGTCGACTAGGG386810.2010772429591775No Hit
TTCGTACCCAGTATAGGCAGTCCGTGAGATCGGAAGAGCGTCGAGTAGGG385040.20015713561955922Illumina Single End PCR Primer 1 (95% over 24bp)
CCAGTAGCGTGAATGGCTCCCACAAGATCGGAAGAGCGTCGTCTAGGCAA278740.1448987117769477Illumina Single End PCR Primer 1 (95% over 23bp)
TTCGCACGCTGCGGTAGTCCGATAACCTCTAGATCGGAAGAGCGTCGAGT275350.14313647229598386No Hit
CGAATTGACTACTCTGAAATTCAGTAACAGATCGGAAGAGCTACGTGAAG251270.13061885379993415No Hit
TTCGTACCCAGTATAGGCAGTCCGTGAGATCGGAAGATCGTCGACTAGGG239140.12431325943294563No Hit
TTCGTACCCAGTATAGGCAGTCCGTGAGATCCGAAGAGCGTCGACTACGG217820.11323038458511425No Hit
CGAATTGACTACTCTGAAATTCAGTAACAGATCGGAAGAGCGTCCTGTAG208900.10859345946116228Illumina Single End PCR Primer 1 (95% over 22bp)
CGAATTGACTACTCTGAAATTCAGTAACAGAACGGAAGAGCTACGTGAAG203780.10593190602678625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)