FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8595623
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCACACGTCTGAACT3291883.8297165894781564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA1747742.0332906643299733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA1128691.3130985386399567Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA1030661.1990521222254629No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA1015901.1818805920175885Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTCGGTAGACCATGTGTCCGGGTTGTAGATCGGAAGAGCACACGTCTGAA566330.6588585841887202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCAGACGTCTGAACT271640.31602130526199207Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCAGAGGTCTGAACT255670.2974420818595697No Hit
GTCGGTAGACCATGTGTCCGGGTTGTAGATCGGAAGAGCACAAGTCTGAA253530.29495244265598897Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTCGGTAGACCATGTGTCCGGGTTGTAGATCCGAAGAGCACACGTCTGAA204140.2374929658967128Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGATGTGAACACGTC188420.21920458819564329No Hit
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCACAGGTCTGAACT185750.21609835610519446Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGCA162060.18853781744499498Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTCGGTAGACCATGTGTCCGGGTTGTAGATCCGAAGAGCACAAGTCTGAA157170.1828488755265325No Hit
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCACACGTCTGAAAT155720.1811619704586858Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GACCAACGTGAATATGTTCCATCGAGATCGGAAGAGCACACGGCTGAACT144300.16787613882088592Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCGGAAGAGCACACGTCTGAA140340.16326914291145622Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGCA135300.15740569357218204Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGCA132620.15428782765367907No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCGGAAGAGCACAAGTCTGAA122520.14253766131902249No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACAAGTCTGAA120700.14042030461317345No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGCA119010.13845418767202797Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACACGTCTGAA115320.13416130512005936No Hit
GACCAACGTGAATATGTTCCATCGAGATCGGCAGAGCACACGTCTGAACT113030.13149715849566693Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGAAGTGAACACGTC100380.11678036600721088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)