FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8595623
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG973581.1326462316925718No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTCTCGGGAA644420.7497071474633078No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG453200.5272450874125122No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCTTCGTCTCGGGAA357830.41629326926041316No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG324850.37792490433794035No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG309060.35955508984049206Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG288940.3361478277956118No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTCTAGGGAA246340.28658772028507995Illumina Single End PCR Primer 1 (96% over 26bp)
ACAACCCGGACACATGGTCTACCGACAGATCGGAAGAGCGTCGACTAGGG233550.27170805420386634No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTGTCGGGAA231990.2698931770274243Illumina Single End PCR Primer 1 (96% over 26bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG210070.24439182593280323No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTCTAGGCAA174580.20310337016874752Illumina Single End PCR Primer 1 (95% over 23bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG160270.1864553622233083No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGCTCGGAAGAGCGTCGACTAGGG148230.1724482332461533No Hit
AACAGCAGAAATAAACCATAGCAAACGCGGAGATCGGAAGAGCGTCGAGT145430.16919076139100098No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTCTCGGCAA138730.16139609659474363No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCTTCGTCTAGGCAA138120.16068643308344258No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTACGG132760.1544507012464367No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTAGACTAGGG132370.1539969819523262No Hit
ACAACCCGGACACATGGTCTACCGACAGATCGGAAGAGCGTCGACTACGG130090.1513444691559879No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCTTCGTCTAGGGAA126750.1474587705859133No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCGTCGTGTAGGGAA124830.14522507559952313Illumina Single End PCR Primer 1 (100% over 26bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGTG109830.12777433351834996Illumina Single End PCR Primer 1 (95% over 22bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGAG95990.1116731154914542No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGTG94220.10961392792587576No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTAGACTACGG91620.10658913263180575No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTCGACTAGGG90840.10568169404358474No Hit
CGATGGAACATATTCACGTTGGTCAGATCGGAAGAGCTTCGTCTCGGCAA89170.10373884475854746No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)