FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579282
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA749120.8731733028474877Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA407380.4748416009638103Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA401190.4676265449719452Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA387400.4515529388123622No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC344930.4020499617567065No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG339730.3959888484840573Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC267950.31232217334737333No Hit
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACACGTCTGAA234120.2728899691139655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC212270.24742163738177622No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA175880.20500550046029495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGGC145200.1692449321516649No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGGC141840.16532852049856853No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGTC133320.15539761952107414No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC129180.1505720408770804No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC117240.1366547923241129No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCAAACGTCTG108900.1269336991137487No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACAAGTCTGAA108370.12631593179942097Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGCA106140.1237166466844195Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACAAGTCTGAA99690.11619853502892201Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGGC86510.10083594408016895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)