FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579282
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT313470.36538022645717905No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG191080.22272260079573095No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT155150.18084263927913782No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT142380.165957943799959No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTACGG126400.14733167647362563No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT126170.14706358877118156No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGGG97830.11403052143524366No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGGG91920.10714183308113663No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGTG91170.10626763405142761No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGTCGAGT89940.10483394764270483No Hit
CTCCAGCATACGAATGCCTGCGTTACAGATCGGAAGAGCGTCGACTAGGG86250.10053288841653649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)