FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7161062
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA231500.3232760727389318Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCACCGCAGTTCCGTATGCGATTTAGATCGGAAGAGCACACGTCTGAA139770.19518054724285308Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA131050.1830035824295335Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA130650.18244500606195002No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA127940.17866065117157204Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACCCACCGCAGTTCCGTATGCGATTTAGATCGGAAGAGCACAAGTCTGAA77470.10818227799172804Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)