FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10128496
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG2588132.5552954752610852Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG1228221.2126380856545729No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG1071961.0583604910344042Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG901900.8904579712525926No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG659810.6514392660075099No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG634270.6262232813242954No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG535140.5283509022464935No Hit
ACGCATGGGTCGTACACAAGTGAATCAGATCGGAAGAGCACACGTCTGAA475090.46906273152499645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCAAACGTCTG439310.43373665744647577No Hit
ACGATACGAGATTACGATAGACTGTCGCAGATCGGAAGTGCACACGTCTG289630.28595558511352526Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCGGAAGAGCACAAGTCTGAA246480.24335301114795327Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCCGAAGAGCACACGTCTGAA216910.21415815339217192Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCCGAAGAGCACAAGTCTGAA196160.19367139997883198No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA185150.18280107925204295Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACACATAGTATTGCAAATTTACCGATATGAGATCGGATGTGAACACGTC146160.14430572910331405No Hit
TTGCCAGATTGACGGCGACGCTGCATGTAGATCGGAAGTGCACACGTCTG140520.13873728142855563Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGAGCACACGTCTG122580.1210248787184198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA113380.1119415952773245Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA111790.11037176694348302No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA107380.10601771477226235Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGAACTAAGGGCTTTAATTGCAGTCATGCAGATCGGATGTGAACACGTC107220.10585974462546067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)