FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10128496
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGAAG534520.527738767927637No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGTAG326300.32216036813363014Illumina Single End PCR Primer 1 (95% over 22bp)
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGTAG326270.3221307487311048No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACGTGAAG271020.2675816824136575No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGAAG237440.2344276978536596No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGAAG231190.2282569889942199No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGAAG215600.21286477281523336No Hit
GATTCACTTGTGTACGACCCATGCGTAGATCGGAAGAGCGTCGACTAGGG199740.1972059820135191No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGTAG183750.18141884046752843No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGTAG165940.16383478850166897No Hit
TCATGTAACCATGATCTACTAACCGACTCGAACGGAAGAGCTACGTGAAG165310.16321278104863743No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCGTCCTGTAG158070.15606463190586242No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGTAG154970.1530039603115803No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACGTGAAG147220.14535228132587502No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGAAG120080.1185565951746439No Hit
GATTCACTTGTGTACGACCCATGCGTAGATCGGAAGAGCGTCGACTACGG109310.10792322966805734No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCGTCCTGTAG108620.10724198340997518No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACCTGTAG105360.1040233416688914No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGAAG104630.10330260287410885No Hit
TCATGTAACCATGATCTACTAACCGACTCGAACGGAAGAGCTACCTGTAG102680.10137734170996364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)