FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9208519
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGATGTGAACACGTC1250731.3582314376502889No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGATGTGCACACGTC583820.6339998864095302No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACACATCGGATGTGAACACGTC582830.6329247949643151No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGAAGTGAACACGTC507000.5505771340646634No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGATGTGAACACGGC372590.4046144662350156No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGAAGTGCACACGTC253760.27557091428057No Hit
ATGCGCGCTGAACGTCTCCTTCATTCGATGAGATCGGATGTGAACACGTC218080.23682418421463866No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACACATCGGATGTGAACACGGC207280.22509591390320202No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACACATCGGAAGTGAACACGTC202920.22036116774043685No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACACATCGGATGTGCACACGTC165000.17918190753583718No Hit
TGTGGGAGCCATTCACGCTACTGGAGATCGGAAGAGCACACGTCTGAACT149910.1627949076284688Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGATGTGCACACGGC131060.14232473213119287No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATCCAGATCGGATGTGAACACGTC120840.1312263133735186No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATCCACATCGGATGTGAACACGGC120230.13056388329111338No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATCCAGATCGGATGTGAACACGGC115530.12545991380372892No Hit
TGACTCTGGCCCGCAGAGGTCTCGACATACAGATCGGAAGTGAACACGGC97410.10578248250342971No Hit
ATGCGCGCTGAACGTCTCCTTCATTCGATGAGATCGGATGTGCACACGTC96120.10438160577178589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)