FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158562
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGATGTGAACACGTC184670.4440717728868777No Hit
GGCTCAGATACTTGATATCTTTGAAGATCGGAAGAGCACACGTCTGAACT150010.3607256546854417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACACGTCTGAA113050.2718487784960282Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGAAGTGAACACGTC92760.2230578743325217No Hit
TAGTCCGCGTTGTGCGGATAAGTTCCCTTCAGATCGGATGTGAACACGTC81770.19663046985953317No Hit
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGATGTGCACACGTC68840.1655379912575549No Hit
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGATGTGAACACGGC61270.14733458344495043No Hit
CATTCCGACCCTTTGAAGCACACTCAAGATCGGAAGAGCACACGTCTGAA55000.13225725623424636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACAAGTCTGAA53840.1294678304663968Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CACCTCCACACATCTACGACCAACGTAGATCGGAAGAGCACACGTCTGAA49680.11946437254031561Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCCGAAGAGCACACGTCTGAA43180.10383396953081378Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)