FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8422186
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA953081.1316301967208988Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA798660.9482811232143293No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA747880.8879879879166762Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA642280.762604862918012Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGACGTCTGAA132380.15718009552389367Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGAAGTCTGAA111800.13274463423154037No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGAAGTCTGAA100430.11924457617060465No Hit
CGTTAAGACACCGCCTTGATCCACTGTCAGATCGGAAGTGCACACGTCTG97500.11576566938797125Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGACGTCTGAA89460.1062194541892093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)