FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8422186
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG621430.7378488197719689No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG295540.35090652236842074No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG204690.24303666530280854No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG197460.23445219566511594No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG196520.233336095878196Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG168400.19994808948650625No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG115140.13671035049570265No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTACGG104030.1235190008864682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)