FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238987
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC303350.4862167528158017No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC271630.43537516587228026No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC243120.3896786449466877No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC213030.34144966161974694No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA153180.2455206269864002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC148420.2378911832962627No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGTC135290.21684610017619846No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG129760.20798248177147988Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA118540.1899987930732986No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA112860.1808947510228824Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGGC100780.1615326334227015No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA97780.15672416050874924Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGCA77600.12437916604089735No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGGC77380.12402654469387418No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGTC75160.12046827473754954No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGCACACGTC70300.11267854861694694No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGCA70160.11245415321429586Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGCA68510.10980949311162212Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)