FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15450007
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA9577736.199175184839722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA5546453.589933648573752Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA5519123.572244336199977No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA5511403.567247574709836Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG1887691.221805271673987Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCAGACGTCTGAA1520470.9841225314655198Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACACGTCTGAA1310070.8479413633922626Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCAGAAGTCTGAA941890.6096372642420163No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCAGACGTCTGAA919670.5952553937354204No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCAGAAGTCTGAA889800.5759220691615221No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGCA823920.53328131178193Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC772930.500278090488891No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC700150.45317131571526154No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGCA631430.40869237146623943No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCAAACGTCTG589180.3813461055389813No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGCA581950.37666649600870733Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC571840.37012280965309596No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGCA565060.3657344621267809Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACAAGTCTGAA537980.34820696197742823Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC496820.32156619734864844No Hit
GTAACGCAGGCATTCGTATGCTGGAGAGATCCGAAGAGCACACGTCTGAA467560.302627694602339Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCACACGTCTG429280.2778510067988966No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC404900.2620710786732977No Hit
GTAACGCAGGCATTCGTATGCTGGAGAGATCCGAAGAGCACAAGTCTGAA348790.22575394302410348No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGGAGACGTCTGAA346760.22444002776179972No Hit
CGATGCAACAAACCAGTCCATACCGAAGCTCGGAAGAGCACACGTCTGAA344560.2230160801868892Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACGGTGCTGGAATATACGTACCTGATAGATCGGAAGAGCACACGTCTGAA343810.22253064351362428Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGCTCCGAAGAGCACACGTCTGAA278980.18056949747660309No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGGC277570.179656876530865No Hit
CGATGCAACAAACCAGTCCATACCGAAGCTCCGAAGAGCACAAGTCTGAA271130.1754885936297634No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGGAGAAGTCTGAA254130.16448536236909148No Hit
CGATGCAACAAACCAGTCCATACCGAAGCTCCGAAGAGGAGAAGTCTGCA248180.16063423142785632No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCACACGTCTG246730.15969572052621075Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CGATGCAACAAACCAGTCCATACCGAAGCTCGGAAGAGCACAAGTCTGAA246200.15935267861043687No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGGAGAAGTCTGAA227520.1472620692016515No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGGAGACGTCTGAA225490.14594815393934774No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA206460.13363100741637204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGCTCCGAAGAGCACAAGTCTGCA185640.12015528536653737No Hit
ACGGTGCTGGAATATACGTACCTGATAGATCGGAAGAGCACAAGTCTGAA178020.1152232487661656Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGCTCCGAAGAGCACACGTCTGCA176890.11449185751177977No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCAGACGTCTGCA176560.1142782653755432Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCAGACGTCTGAA172990.11196758681080209Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCAAACGTCTG167620.10849186023022513No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGCACACGGC158700.1027184000628608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)