FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15450007
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG2388801.5461481667937107No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTACGG1742491.127824731729895No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGTG1273910.8245368432519157No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGGG1221050.7903232665202029No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGGG1181840.764944637241912No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTACGG1137860.736478630721656No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTACGG1053180.681669594065556No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTAGACTACGG921630.5965240015748859No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGAGTAGGG869830.562996508674721Illumina Single End PCR Primer 1 (95% over 24bp)
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT856630.5544528232252581No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTCGACTAGGG807660.5227570447055461No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTCGACTACGG806340.5219026761605998No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTAGACTACGG740560.4793266436707764No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTAGACTACGG706730.45743021346203916No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCCTACACTACGG647000.41877003680321956No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGTG635150.41110013736563356No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTAGACTAGGG617970.3999804013033781No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGTG601050.3890289499545211No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTAGACTAGGG514660.3331131176833771No Hit
CTCCAGCATACGAATGCCTGCGTTACAGATCGGAAGAGCGTCGACTAGGG508350.3290289771389748No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCCTACACTACGG505790.3273720199608971No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTAGACTACGG459830.29762446062322173No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGAGTAGGG449240.29077009479672083No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGAG437510.2831778652268572No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTCGACTAGTG431080.2790160548147324No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCCTAGACTACGG429540.27801929151229515No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGAGTAGTG422160.2732425946473681Illumina Single End PCR Primer 1 (95% over 22bp)
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTAGACTAGGG401210.25968273024083416No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT371330.24034293317795907No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTAGACTAGGG363190.23507432715079027No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT357790.23157918310328274No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCCTAGACTACGG337020.2181358234983324No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGAGTACGG324180.20982514765203666Illumina Single End PCR Primer 1 (95% over 21bp)
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCCTAGACTACGG322140.208504759900756No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT320440.2074044367746888No Hit
CTCCAGCATACGAATGCCTGCGTTACAGATCGGAAGAGCGTCGACTACGG311160.20139796700415735No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGAGTAGGG309720.20046592859148865No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCCTAGACTACGG287650.18618114541954575No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACGTGAAG272080.17610348008256566No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCCTACACTACGG244250.15809054325994804No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGAG235800.1526212900744964No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGAGTAGTG226920.14687371986303954No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGTCCTGTAG218510.14143035663349537Illumina Single End PCR Primer 1 (95% over 22bp)
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGAGTAGTG207100.13404524671089144No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGCTCGGAAGAGCGTCGACTAGGG201690.13054363017440704No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGACGAGT200210.1295857018058309No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGAG199490.12911968259949658No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGCTCCGAAGAGCGTCGACTAGGG197620.12790932716082265No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGTCGAGT197250.1276698450686786No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCCTAGACTAGGG192190.12439476564638449No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGATCGTCGAGTAGGG192030.12429120582275463No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACCTGTAG188630.12209055957062026No Hit
CTCCAGCATACGAATGCCTGCGTTACAGATCGGAAGAGCGTCGACTAGTG186900.12097081897762248No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGACGAGT185280.11992227576337021No Hit
CTCCAGCATACGAATGCCTGCGTTACAGATCCGAAGAGCGTCGACTAGGG181960.11777340942305074No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCCTACACTACGG177230.1147119221369932No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGAGTACGG168210.10887373707986023No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGTCTAGGG167680.10853069516408634Illumina Single End PCR Primer 1 (95% over 24bp)
CTCCAGCATACGAATGCCTGCGTTACAGATCGGAAGAGCGTCGAGTAGGG158520.10260189526127723Illumina Single End PCR Primer 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)