FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6590431
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG465880.7069036911242982Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG215230.3265795514739476No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG151090.22925662980160175No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG137010.20789232145818687Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CCGTTCTCTGGCTCACGACAATTTGTCAAGATCGGAAGTGCACACGTCTG91550.138913524775542Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG90230.13691062086834685No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG80870.12270821134459946No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG75650.11478763680250959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)