FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7008394
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG2230973.1832827891810878Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG1175021.6765895296411704No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG751211.0718718154259022No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG703521.0038248420394171Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG511600.7299817904073316No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG487770.6959797066203756No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG402870.5748392570394872No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCAAACGTCTG320280.4569948550267008No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGGACACGTCTG94650.1350523386670327No Hit
CAATCCGGTGGTCTCGAGAGAGCTCTCTAGATCGGAAGTGCACACGTCTG93350.13319742012221344Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCTGTGTTTAGTGGCAGGAAGCTGCTGAGATCGGAAGTGCACACGTCTG80140.11434859398601162Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGAGCACACGTCTG74660.10652939888938892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)