FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7008394
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGAAG439010.6264059925854626No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGTAG265100.3782606971012189Illumina Single End PCR Primer 1 (95% over 22bp)
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGTAG258670.3690859846064591No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACGTGAAG208420.2973862485470994No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGAAG195060.2783233933480338No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGAAG187740.2678787750802823No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGAAG179000.25540801501742055No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGTAG149880.21385783961346921No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACGTGAAG135320.19308275191149357No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCGTCCTGTAG134140.19139905661696532No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGTAG130410.18607686725375314No Hit
TCATGTAACCATGATCTACTAACCGACTCGAACGGAAGAGCTACGTGAAG124670.1778866884481666No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGTAG121260.17302109441906377No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGAAG105860.15104744396505107No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCGTCCTGTAG84070.11995615543304214No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGAAG82490.11770171597087721No Hit
TCATGTAACCATGATCTACTAACCGACTCGAACGGAAGAGCTACCTGTAG78920.11260782427471971No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACCTGTAG77650.11079571154247322No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACCTGTAG76820.10961141739462706No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGTAG75360.10752820118275315No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACCTGAAG74450.10622975820137966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)