FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30715412
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA11861283.86167048646458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA8916232.9028521577376205No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA8868902.887442955347628Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA7591272.4714856502657363Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGCA1490870.48538173604833945No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGCA1400230.4558721204846609Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGCA1373170.44706221098385396Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGATGTGAACACGTC1296930.4222407955979884No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGCA1151280.3748216042161505Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGACGTCTGAA892760.2906553882461352Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACAAGTCTGAA698050.22726375931405382No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCGGAAGAGCACAAGTCTGAA639080.20806492844699592No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGAAGTCTGAA637490.20754727301069573No Hit
CGTTAAGACACCGCCTTGATCCACTGTCAGATCGGAAGTGCACACGTCTG614520.2000689425881704Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCGGAAGAGCACACGTCTGAA610490.19875689767729632Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGAAGTCTGAA610040.19861039142173967No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGACGTCTGAA579680.18872610271351725No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACACGTCTGAA571060.18591969399596528No Hit
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGAAGTGAACACGTC534120.17389315826204774No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACAAGTCTGCA509190.16577671170420893No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGGAGAAGTCTGCA428080.13936977306376355No Hit
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGATGTGCACACGTC405640.13206399445333827No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCCGAAGAGCACACGTCTGCA392310.12772415359429332No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGCTCGGAAGAGCACAAGTCTGCA370240.12053883568288128No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGGAGACGTCTGAA354120.11529065603938504No Hit
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGATGTGAACACGGC341520.11118848088379867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)