FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30715412
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG6378882.0767684965449917No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG3337141.0864708570407586No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG2282150.7429983358191646No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG2173620.7076642826734669No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG2149540.6998245701539019No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG2068740.6735185580450622Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTACGG1479600.4817125682702873No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG1299850.42319145841182265No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTCGACTAGGG1054510.34331624788233345No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTAGACTAGGG1005410.3273307875538183No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTAGACTACGG835650.2720621165687115No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTCGACTACGG783830.2551911073177205No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGTG774090.2520200608085609Illumina Single End PCR Primer 1 (95% over 22bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGTG769220.25043453755398104No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTAGACTACGG751740.2447435834492469No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGTG719120.23412350776867324No Hit
AACAGCAGAAATAAACCATAGCAAACGCGGAGATCGGAAGAGCGTCGAGT701770.22847487769332217No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGAGTAGGG699800.22783350586344078No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGAG667640.21736319213299174No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTAGACTAGGG643910.20963742892330406No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGCTCGGAAGAGCGTCGACTAGGG587340.19121996475254835No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTACGG522560.17012957534152562Illumina Single End PCR Primer 1 (95% over 21bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGTCTAGGG496210.16155082015504138Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCCTACACTACGG487010.15855558115254975No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGAGTAGGG478620.1558240534100601No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCCTACACTACGG470270.1531055484458421No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTAGACTACGG454800.14806898894926104No Hit
AACAGCAGAAATAAACCATAGCAAACGCGGAGATCGGAAGATCGTCGAGT418090.13611733419040578No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTAGACTAGGG397170.12930642115430518No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTCGACTAGTG378950.12337454565154457No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCCTAGACTACGG371820.12105323542461355No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTAGACTACGG368490.11996908913349429No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGATCGTAGACTAGGG365470.11898586937398072No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGCTCCGAAGAGCGTCGACTAGGG325520.10597936957511753No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCCTAGACTACGG324640.10569286845314008No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCCTCGACTAGGG321570.10469337022078688No Hit
AACAGCAGAAATAAACCATAGCAAACGCGGAGATCGGAAGAGCGACGAGT318230.10360596823509968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)