FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24363536
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC556910.22858340431372526No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC389300.15978797166388325No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG270860.11117433856891708Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC265240.10886761264867299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)