FastQCFastQC Report
Sat 5 Aug 2023
EGAF00007985569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33761984
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGAGAGTCTCTTATTAATGAGAGATCGGAAGAGCACACGTCTGAACT9345082.7679297519956174Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTTGAGAGTCTCTTATTAATGAGAGATCGGAAGAGCAGACGTCTGAACT3087310.9144338200029951Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTTGAGAGTCTCTTATTAATGAGAGATCGGAAGAGCAGAGGTCTGAACT1177090.34864361051767573No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA1027830.30443412330270636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTAAGGACAGTCACCTAACAGAGATCGGAAGAGCACACGTCTGAACT861820.255263434755493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAGGTTCCATTGTGCTGCAGGTAAGATCGGAAGAGCACACGTCTGAACT699240.20710868176467356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA595390.17634923350476087Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TACCACGAATCGGCTTAGACATTGAGATCGGAAGAGCACACGTCTGAACT577280.1709852122434511Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA569990.1688259789472088No Hit
TCTTTGGTTACGCCATCGGTTGGGATAGATCGGAAGAGCACACGTCTGAA567820.16818324420744943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA567510.16809142495891236Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTTGAGAGTCTCTTATTAATGAGAGATCTGAAGAGCACACGTCTGAACT560200.1659262678401838Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AAACCTAATGCGGAGGTAGCCATTTAAAAGATCGGAAGTGCACACGTCTG530520.15713531527057178Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG504250.14935437443486735Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGTCCGCGTTGTGCGGATAAGTTCCCTTCAGATCGGATGTGAACACGTC360940.10690722440956076No Hit
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACACGTCTGAA341560.10116704042037339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)