FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33761984
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTAATAAGAGACTCTCAACAAGATCGGAAGAGCGTCGTCTCGGGAA1973090.5844117454708823No Hit
CTCATTAATAAGAGACTCTCAACAAGATCGGAAGAGCGTCGTCTAGGGAA721530.21371078192561196Illumina Single End PCR Primer 1 (96% over 26bp)
CTCATTAATAAGAGACTCTCAACAAGATCGGAAGAGCTTCGTCTAGGCAA656730.19451759707012478No Hit
CTCATTAATAAGAGACTCTCAACAAGATCGGAAGAGCGTCGTCTAGGCAA647050.19165046698677424Illumina Single End PCR Primer 1 (95% over 23bp)
CTCATTAATAAGAGACTCTCAACAAGATCGGAAGAGCTTCGTCTAGGGAA494670.1465168634639481No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG364470.10795277907838592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)