FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18816717
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA1454050.7727437256988028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA800380.42535581525725236Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA791130.42043997366809527Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA771590.41005559046245954No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGCA223830.1189527376109233Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)