FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23727052
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGAAG893320.37649852160310515No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGTAG563020.23729033004184422No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGTAG557020.23476157088541805Illumina Single End PCR Primer 1 (95% over 22bp)
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACGTGAAG402100.16946900946649418No Hit
CAGGAATTAATCACTCAGTTGGCCGTAAGCAGATCGGAAGAGCGTCGAGT371960.15676620930404672No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGAAG360970.15213436544919276No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGAAG356650.15031365885656592No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGAAG355890.1499933493634186No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGTAG302350.127428388490909No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGTAG286990.12095476505045802No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGTAG276790.11665587448453352No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACGTGAAG258580.10898109044478008No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCGTCCTGTAG255320.10760713130312186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)