FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29420757
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAGATACTTGATATCTTTGAAGATCGGAAGAGCACACGTCTGAACT698980.23758056259395366Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTCCTCACGCGGTCCATAGTCCAAATAGATCGGAAGTGCACACGTCTG521260.17717423110493044Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACACATAGTATTGCAAATTTACCGATATGAGATCGGATGTGAACACGTC349810.11889904804284948No Hit
CATTCCGACCCTTTGAAGCACACTCAAGATCGGAAGAGCACACGTCTGAA305660.10389263607323224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCCTCACGCGGTCCATAGTCCAGATAGATCGGAAGTGCACACGTCTG296440.1007587942077765Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)