FastQCFastQC Report
Thu 9 May 2024
EGAF00007985595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26964057
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG3345171.2406033706277952Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG1680330.6231740275582417No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG1081320.4010227392710229No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG978860.36302400636521426Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG706970.2621897735937882No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG625170.2318530924333827No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG539330.2000181204186002No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCAAACGTCTG408810.15161294162818303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)