FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26964057
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGAAG789170.29267480038334No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGTAG523990.194329065540842Illumina Single End PCR Primer 1 (95% over 22bp)
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGTAG501120.1858474041944059No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGAAG406030.15058193950561669No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGAAG338160.1254113948802289No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACCTGTAG323980.1201525423269948No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGAAG313720.11634747693939379No Hit
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACGTGAAG310380.11510879093602272No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCGTCCTGTAG287750.10671613696707435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)