FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28919943
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG4350231.504231872102929Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG2160170.7469482218550707No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG1471820.508929080531037No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG1294710.4476876043635356Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG916050.3167537363403517No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG825270.2853636329781148No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG742740.2568262323338604No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCAAACGTCTG543200.18782886259492282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)