FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28919943
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACGTGAAG854570.29549504990379827No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGTAG535280.18509026798565958No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGTAG503510.17410476915531958Illumina Single End PCR Primer 1 (95% over 22bp)
TCATGTAACCATGATCTACTAACCGACTAGAACGGAAGAGCTACGTGAAG378890.13101339791713973No Hit
TCATGTAACCATGATCTACTAACCGACTCGATCGGAAGAGCTACGTGAAG343510.11877962553384008No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTACCTGAAG333820.11542899652326424No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCGTCCTGAAG324150.11208528315564109No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAAGAGCTTCCTGTAG306520.1059891438928493No Hit
TCATGTAACCATGATCTACTAACCGACTAGATCGGAATAGCTACGTGAAG293730.10156659022460728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)