FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15841982
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAAATCGCCACGATTCAGTTCGCTAGATCGGAAGTGCACACGTCTG604970.3818777221183562Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACACGTCTGAA421600.266128316519991Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACAAATCGCCACGATTCAGTTCGCTCGATCGGAAGTGCACACGTCTG312800.1974500412890256Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CACCTCCACACATCTACGACCAACGTAGATCGGAAGAGCACACGTCTGAA304500.1922107978660751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACACGTCTGAA300840.18990048088679812Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCACACGTCTG210440.13283691396695185Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGTGATAGGGAAGACATTCACTAAAGAGATCGGAAGAGCACACGTCTGAA197820.12487073902747775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACAAGTCTGAA159110.1004356651838135Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)