FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15841982
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGACTAGGG260840.16465111499306084No Hit
AGCGAACTGAATCGTGGCGATTTGTCGGAGATCGGAAGAGCTACGTGAAG184680.11657632233138505No Hit
AGCGAACTGAATCGTGGCGATTTGTCGGAGATCGGAAGAGCGTCCTGTAG181010.11425969301063466Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)