FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16213663
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCGGCCGTCTTTATTCGATTCAAGGAGATCGGAAGAGCTACGTGAAG366090.2257910504245709No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAAGAGCTACGTGAAG334810.2064986795395957No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGATCGGAAGAGCGTCCTGTAG303250.18703361479759387Illumina Single End PCR Primer 1 (95% over 22bp)
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAATACCTACGTGAAG250060.15422794960028466No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGATCGGAAGAGCTACCTGTAG241280.14881276365494953No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAATAGCTACGTGAAG230390.1420962061441637No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAAGACCTACGTGAAG208470.1285767441940788No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGATCGGAATAGCTACGTGAAG199140.12282233817244134No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAATACCTACCTGTAG194790.1201394157507776No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAAGAGCGTCCTGTAG190700.11761685191063857No Hit
GGATCGGCCGTCTTTATTCGATTCAAGGAGAACGGAAGAGCTACCTGTAG184280.11365722847452794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)