FastQCFastQC Report
Tue 8 Aug 2023
EGAF00007986004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007986004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274674467
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTGGATCCTGGGTGATGTCTTCATCCGCCAGTACTTTACCGTCTTCG4397800.16010953067581632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4383420.15958600185433325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCATCCGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCG4016440.146225458953926No Hit
TTTGGATCCTGGGTGATGTCTTCATCCGCCAGTACTTTACCGTCTTCGAC3717160.13532965188205862No Hit
ACCGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATCCGCCAGTA3696060.13456146981437486No Hit
GTGGCTTCCAGGGCATGAACCTCCCCACCGAATCTGGAGAGCTTTGGATC3386760.12330086727719036No Hit
CACCGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATCCGCCAGT3355800.12217371482147993No Hit
CAAACAACCAGGTCGGCCTGGCCCCCGTGGCTTAAGCCTAAGTCTCTTCA3269210.11902125580533118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA3236100.11781582887352977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCATCAGTGGCTTCCAGGGCATGAACCTCCCCACCGAATCTGGAGAG2956810.10764779239564339No Hit
CGAATCTGGAGAGCTTTGGATCCTGGGTGATGTCTTCATCCGCCAGTACT2926830.10655631853833723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCAAAACCAACCCGGTC2925390.1065038928427228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGGGCATGAACCTCCCCACCGAATCTGGAGAGCTTTGGATCCTGGGTGA2889810.1052085412802494No Hit
CCGTCTTCGACAGGGCAAACAACCAGGTCGGCCTGGCCCCCGTGGCTTAA2820310.10267827333219144No Hit
CTTTGGATCCTGGGTGATGTCTTCATCCGCCAGTACTTTACCGTCTTCGA2811440.10235534560989973No Hit
ATCCGCCAGTACTTTACCGTCTTCGACAGGGCAAACAACCAGGTCGGCCT2775550.10104870796017601No Hit
GGATCCTGGGTGATGTCTTCATCCGCCAGTACTTTACCGTCTTCGACAGG2770210.10085429600560579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)