FastQCFastQC Report
Wed 31 May 2023
EGAF00007986131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007986131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158549772
Sequences flagged as poor quality0
Sequence length10-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGATAGTTCAGACGGTTTCCATTTCCTGAGCGTCTGAGATGTTAG5600960.3532619397270404No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT2558300.16135627113989165No Hit
GCGGCTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATG2388780.15066436046341333No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC2384700.15040702802145942No Hit
NNNNNNNNNN2344710.14788479166024912No Hit
GGGGCGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTTTTAGGGGCTC1861460.11740540377440593No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT1799520.11349874410415425No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA1768560.1115460449857979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)