FastQCFastQC Report
Thu 6 Jun 2024
EGAF00007986249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007986249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388989
Total Bases473.3 Mbp
Sequences flagged as poor quality0
Sequence length20-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTT169180.499204925126638No Hit
GGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAC112540.3320754360666264No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT85060.2509893068404766No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC79190.23366850703852976No Hit
CCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC74550.21997710821722938No Hit
ATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGA63800.1882567337928804No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC60380.17816522862718057No Hit
CCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC59930.17683739900011478No Hit
ACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG59150.1745358276465341No Hit
GGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAG44460.13118956715409816No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG42590.12567169737051376No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG37500.11065246892214757No Hit
TCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTG37290.11003281509618355No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT35290.10413135008700235No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT34790.10265598383470703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)