FastQCFastQC Report
Thu 6 Jun 2024
EGAF00007986253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007986253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41956661
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTCTATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGG1557010.37109959727252845No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA1364640.32524990489591155No Hit
CTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACT1155220.27533649543751826No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC1084100.25838567087118774No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT1054060.2512259018895712No Hit
CCACACATTGAGCCGTCCACCCCCTGCTCAGGTGCCCAGGGAGGTGCTCA1053580.2511114981242192No Hit
GCCACCGTGAGTTCAGAACGCAGGGTGGGAGCCACCAGCTTCAGCTCTCG963910.22973944470938715No Hit
ATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTAT949320.2262620469250401No Hit
GTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTT868640.20703268069878106No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG854670.20370305444468045No Hit
TGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGAC802160.1911877591975205No Hit
TAGCTGCCTCACTGCGGCTGAGGTCCATCCTGCTGGACGCTATGTCCTCT730380.17407962945383096No Hit
GGACGTCAATGTCCGCGGGCCTGATGGCTTCACCCCGCTCATGATCGCCT696440.16599032987872891No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA663660.15817750606989436No Hit
AAAAGGGACTTGCTGATCATAAAACCCGTCCATGGTGCTTTCAGCGTCTC652030.155405598171885No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA639390.15239296568428073No Hit
CTCTGACAGCGAAGACTCCGAAACAGAGATGGCTCCGAAGTCAAAAAAGA576130.13731550277559026No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC542870.12938827520140367No Hit
AATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAG531880.1267689056571971No Hit
GCCGCTTGTACTTCTGCCGCCCACCCCGGACGCGGTCCAGGCGCACTCCC499700.11909908655505261No Hit
CTTGCTTCTCACTGCTGCGGGCTGTGGATTTAAGCATCTTGACGGCCACT493270.1175665527816906No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC480280.11447050088185046No Hit
GTTGTCCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGG467690.11146978545313699No Hit
CACAGGACTGCAGCTCCCCAGCACGCTGCCTGTGTCAGGGAATCTGCTTG460130.10966792614884202No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT458980.10939383379435269No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG457910.10913880873408874No Hit
GTGAGGGCAGAGGCATGCCACCACCACTCCGTGGAGGTCCAGGAGGCCCA439800.10482245000382658No Hit
GCCGCTTGTACTTCTGCCGCCCACCCCGGACGCGGTCCAGGCGCACTCCT425120.101323601513476No Hit
GGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGAATGGGGTAAGA419860.10006992691815968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)