FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217449738
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA7276202033.46153491341525No Hit
GCTCAACC5287548524.316187035369065No Hit
TTGTTGAT4732384721.76311980656422No Hit
CAAAGTGG4127141918.979751081603972ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGTTGAT6003100.27606839425118096No Hit
GCTCAACA5211440.2396618201489854No Hit
CAAAGTGA2864770.13174400789574647No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)