FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34273387
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA3823941.1157169847263708No Hit
CTCTACGAGCTCCCAGAGCCCCGAGCTT1524690.44486119799014906No Hit
TGACTTTTACTTCCTCTAGATAGTCAAG1477290.43103122548115835No Hit
TGGCGCAAGTCCTCCTAACTGTCGATTT1412490.41212442762076595No Hit
GATCGCGGTCTCGTTCGCTATTTGCATT1341430.39139113972015666No Hit
TTGACTTTTACTTCCTCTAGATAGTCAA1327870.3874347172049264No Hit
TTGAACGCAAGAAGAGCATATCTGGGTT1300970.3795860619202882No Hit
CGTCACTTCCAAACACCAGACTGCTATT1295330.37794047025466143No Hit
GTGAAGGTCCTCAACCGGCTTTCGCGTT1279600.3733509034283656No Hit
ACCTTTAAGTTTCAGCTTTGCAACCATA1249010.3644256110433439No Hit
TTGAACGCAAGAAGAGAGAGTCCGTCTT1205070.35160516817319515No Hit
CGTCACTCCGATCTCGTCACTTCCAAAA1195790.34889752798578094No Hit
GATCGCGGTCTCGTTCCTCATGAACATT1195230.34873413590550595No Hit
ATGCGATTCGCAAACTGAAAATACACTT1186740.3462569952599082No Hit
TGGACGCAGATCCCAACGGTAAACTCTT1179620.3441795816678404No Hit
CCTTACGGTTTGACTGGAGACCGATGTT1179590.3441708285206828No Hit
GATCGCGGTCTCGTTCACCCCACTCTTT1159760.33838499824951646No Hit
TTGAACGCAAGAAGAGCAGCCTATGTTT1159330.338259536473591No Hit
CCTTACGGTTTGACTGAAACTCGGTTTT1127310.32891701074072427No Hit
GGAAAGCAGGTGACCACACCGCGTGCTT1121940.3273501973995158No Hit
CAGCATAGTCCGAGTCTCAGTTCCTCTT1109270.32365345158329406No Hit
ATGCGATTCGCAAACTCTGAGTCGCCTT1108970.32356592011171814No Hit
CCTTACGGTTTGACTGAGCGCCTCGTTT1102310.3216227214427334No Hit
GTGAAGGTCCTCAACCTCGGCACTGCTT1098140.3204060339878285No Hit
TTGAACGCAAGAAGAGTTCAAACACCTT1091910.31858829709476916No Hit
CTCTACGAGCTCCCAGACTATCGACTTT1091290.31840739872017904No Hit
CCTTACGGTTTGACTGCCTCGAACTATT1087970.3174387171014058No Hit
GATCGCGGTCTCGTTCGCCGCACAAATT1087450.3172869958840076No Hit
GTGAAGGTCCTCAACCTGCTGCAGCGTT1079210.3148827981313898No Hit
CCTTACGGTTTGACTGGGCGACCATATT1067210.31138153926835416No Hit
CCATGTCTCACTGGGCAATTTGAGTGTT1067090.31134652667972385No Hit
AAAGTAGAGGTGCACACCATCCATGCTT1062720.310071484910435No Hit
GCATGTATCCGTCATCGGTCGGAAGATT1057100.3084317286762467No Hit
GGGAGATCATAACCTGCATAAATCCCTT1053580.30740469274308957No Hit
TCGTAGAGTCCGCTGAACCTCTTCAGTT1042790.3042564774820767No Hit
GTGAAGGTCCTCAACCGCATTGTCTTTT1038390.30297268256563026No Hit
TTCTCCTTCTCTTATGGCGCCCAGGCTT1036370.3023833039903526No Hit
GGGAGATCATAACCTGTGTCTGCGGCTT1033010.30140295150870267No Hit
AAAGTAGAGGTGCACATTGACAATGATT1032640.3012949960270924No Hit
TGGACGCAGAGCCCAAGTAATCATGTTT1023530.2986369570069045No Hit
GCACATATCATGTCCCGGTCATGAGTTT1020000.2976070033580282No Hit
AAAGTAGAGGTGCACATACCCGAATATT1013410.2956842286990778No Hit
CTCTACGAGCTCCCAGCACACGACTATT1006220.2935863910969756No Hit
GGGAGATCATAACCTGCTTGGGTCCATT1003970.2929299050601564No Hit
GGGAGATCATAACCTGTGCAACCACATT1003090.2926731460768672No Hit
GGGAGATCATAACCTGCCGCCATTATTT998850.2914360346119279No Hit
GTGAAGGTCCTCAACCACCTCTGCGGTT997980.29118219334435785No Hit
GCATGTATCCGTCATCCGTTAGCGTGTT996220.2906686753777793No Hit
GCATGTATCCGTCATCGGCGCGTCTGTT992980.28972333548475965No Hit
AAAGTAGAGGTGCACAAAACGTGTGATT989060.2885795909228347No Hit
AAAGTAGAGGTGCACAGTCAACCCATTT986940.2879610351903651No Hit
CCTTTCTGTTAAGAACCGACGTCTTATT982730.28673267687258336No Hit
GCATGTATCCGTCATCCCTGATAGTCTT977440.2851892052571285No Hit
GCACATATCATGTCCCTTGCTAGTTATT973080.2839170812035589No Hit
GGGAGATCATAACCTGAGCGGGCAACTT971430.2834356581098915No Hit
AAAGTAGAGGTGCACATCCGCTATCTTT952350.27786865651766485No Hit
GCACATATCATGTCCCTATGACTTCTTT945860.2759750590159064No Hit
TGGCGCAAGTCCTCCTGTCTCTCGCCTT943990.2754294461764167No Hit
GTGAAGGTCCTCAACCACCACGGAAGTT942110.2748809156212078No Hit
CAGCATAGTCCGAGTCCAGTATCTCTTT935160.2728531031963663No Hit
GATCGCGGTCTCGTTCGATGGTCTCATT925810.2701250389989177No Hit
GCATGTATCCGTCATCAAGCTGCGGGTT921380.26883249093531375No Hit
CTCTACGAGCTCCCAGCATGACCCCCTT920800.26866326342360036No Hit
CACATTTAGCCCTAATGAGACCAATGTT906520.26449676537658795No Hit
TCGTAGAGTCCGCTGAGCTTCAACAATT902680.2633763625404165No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG900710.2628015725437349No Hit
GCATGTATCCGTCATCGGTCTATTGTTT897440.2618474795035577No Hit
GTGAAGGTCCTCAACCAGACAAAGACTT896850.26167533427612505No Hit
GGGAGATCATAACCTGCCCTCTTGCCTT893660.26074458296170144No Hit
GTGAAGGTCCTCAACCCAGCTTGGCCTT890550.25983717337303136No Hit
CAAGATCAGGTGATATACCCCGGCTTTT883510.2577831015067171No Hit
GTGAAGGTCCTCAACCCCAATAATGCTT869410.25366912234265027No Hit
TCGTAGAGTCCGCTGATGGGGCCGGCTT857310.2501386863224227No Hit
ATCTACTTCATGTCCCAGGCTGCGGTTT845970.24682999669685401No Hit
CCTTACGGTTTGACTGTAGCCGCTGATT837470.24434993833553714No Hit
CAACCTCCATTATCTCAAAGTAGGCATT823930.24039935125174527No Hit
GATCGCGGTCTCGTTCGCTGAGACCATT817240.23844739943560292No Hit
TCGTAGAGTCCGCTGACAAGATAAGTTT806160.23521457041873334No Hit
CACCTTGTTACGACTTTTACTTCCTCTA795980.23224433581659146No Hit
CTCTACGAGCTCCCAGTTTTACCGCGTT792310.2311735341476464No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC780150.22762559183310363No Hit
GTGAAGGTCCTCAACCTTAAAAATATTT778650.22718793447522417No Hit
GTGAAGGTCCTCAACCTCGGTTCTTCTT775880.2263797272210068No Hit
GATCGCGGTCTCGTTCTCTAGTACCTTT775440.22625134772936215No Hit
TGTTCCGTTGGTCAAGTTATTGGATCAA773990.22582827895007868No Hit
GATCGCGGTCTCGTTCCCGACAACCGTT772160.22529433697346574No Hit
CCTTACGGTTTGACTGAGAAAGCGCGTT765010.22320817023424033No Hit
CAACCTCCATTATCTCCCTGGATGGTTT729310.21279192511670936No Hit
CCTTTAAGTTTCAGCTTTGCAACCATAC721990.2106561572102576No Hit
CTCTACGAGCTCCCAGGTTTTTATCTTT708980.20686020905958316No Hit
GCATGTATCCGTCATCTTTGTGGACTTT685560.20002691884522533No Hit
CAACCTCCATTATCTCGGCATACAGTTT677960.19780945489863608No Hit
GTGAAGGTCCTCAACCCGGCGCTCGTTT674580.1968232669855477No Hit
CACATTTAGCCCTAATCCAAACATCGTT668070.19492383405235086No Hit
CAGCATAGTCCGAGTCATGCGAAGTTTT662470.19328991324960093No Hit
CACATTTAGCCCTAATCGCATAGCCCTT655050.19112496818595723No Hit
TTGACTTCCTCTAGATAGTCAAGTTCGA652660.1904276341290693No Hit
CAGCATAGTCCGAGTCCTTGGGGTCTTT647190.18883164363066887No Hit
GGGAGATCATAACCTGCAACCCCTGTTT645980.18847860002864614No Hit
TCGTAGAGTCCGCTGATTCCTTCGTCTT637500.18600437709876763No Hit
CAACCTCCATTATCTCAGCGCGATACTT634450.18511447380441273No Hit
GATCGCGGTCTCGTTCCAAATCTTGCTT621590.18136229138952623No Hit
GATCGCGGTCTCGTTCTTGCCCTGCATT611280.17835412648303478No Hit
GCACATATCATGTCCCAGTAACCATGTT611230.1783395379044388No Hit
GTGAAGGTCCTCAACCAGTGTATGGCTT603160.17598494131904732No Hit
CACATTTAGCCCTAATACTAACCTGCTT574370.16758483776348104No Hit
CTCTACGAGCTCCCAGTCATGCCGCTTT561640.16387058565294407No Hit
GTGAAGGTCCTCAACCGCTCTGCGTCTT555180.16198574129834323No Hit
GTGTGCGTCGATGAGGCATCCCAGGCTT551610.16094411678659015No Hit
CTCTACGAGCTCCCAGGAATGTGCCGTT550810.16071069952905442No Hit
ATGCGATTCGCAAACTCAACTACCTTTT522610.1524827412009207No Hit
TCGTAGAGTCCGCTGATAAATAGCGCTT515410.15038198588309934No Hit
GTGAAGGTCCTCAACCAAAATTCGATTT510870.14905734294658418No Hit
CCTTACGGTTTGACTGGGTACGTGCGTT506520.14778813660873377No Hit
TCGTAGAGTCCGCTGACTCTTCGTGATT503790.14699160021739316No Hit
CGTCACTTCCAAACACGGGGGTCGCATT500940.1461600512374222No Hit
CCTTACGGTTTGACTGCAGCCAGGGATT487550.1422532298894183No Hit
ACCAGTACATGAAGTATGTACAACTCTT474070.1383201490999416No Hit
CACATTTAGCCCTAATACACGCATATTT445420.12996089356444404No Hit
CTAAAGGTTAGAACACGACAGACGCTTT429840.12541509247393612No Hit
CTCTACGAGCTCCCAGTACTCGCTTATT426970.12457770806252677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)