FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87686703
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA2430498827.71798592997618No Hit
GCTGAATT2167752224.72156126111846No Hit
ATGCTCCG2166428424.706464331313725No Hit
TGAAGTAC1870586621.33261413649No Hit
CACTCGAA2459750.2805157356640493RNA PCR Primer, Index 47 (100% over 8bp)
GCTGAATA1616940.18439968030272502No Hit
CACTCGCA1159020.13217739524315333No Hit
ATGCTCCA1085120.12374966361775515No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)