FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95517220
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAT3095146332.40406598935773No Hit
ATGCCTTA2249386623.549540072460232No Hit
TAATGACC2214725323.186659955136886No Hit
CGTATCGG1883772919.721814558673294No Hit
ATGCCTAA1300290.13613147451318203Illumina PCR Primer Index 3 (100% over 8bp)
CGTATCGA1217030.1274147216596128No Hit
TAATGACA1080420.11311258849451439No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)