FastQCFastQC Report
Wed 6 Sep 2023
EGAF00007987381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98759867
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2574019726.063418048142978Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52282465.293897368249797Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18575841.8809097829181969Clontech SMART CDS Primer II A (100% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7217070.7307695138957608Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7041220.7129636980981353Clontech SMART CDS Primer II A (100% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6966990.705447487085012Clontech SMART CDS Primer II A (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3631730.36773338303503383No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA2423790.24542256623330608Clontech SMART CDS Primer II A (100% over 26bp)
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC2078940.21050453622016319No Hit
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTAT1759060.17811486117128933No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT1728030.17497289663219168No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG1590730.1610704882784016No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA1523010.1542134519075446Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG1505960.15248704213018027Clontech SMART CDS Primer II A (100% over 26bp)
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1486750.15054192002911465No Hit
GTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCC1358040.13750929818485885No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA1199870.12149368325901047No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG1190870.1205823819102551No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG1185240.12001231228875593No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC1162650.11772494590337997No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG1105620.11195033302343349No Hit
GGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTC1101340.11151695860424761No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC1095290.11090436158647317No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG1091330.11050338899302081No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC1059430.10727333199021015No Hit
GCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTAT1044930.10580512426165985No Hit
GTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGC1034300.10472877611307435No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA1028250.10411617909529991No Hit
GAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCC1019450.103225128887628No Hit
GCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTT1018400.10311881039693988No Hit
GCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTG991820.10042743374694905No Hit
ATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAAC991600.10040515749175724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)