FastQCFastQC Report
Wed 6 Sep 2023
EGAF00007987387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97566113
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2528442425.91516995250185Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51450215.273368838625354Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18271421.8727219357401272Clontech SMART CDS Primer II A (100% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7113170.7290615338954827Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6954840.7128335634320084Clontech SMART CDS Primer II A (100% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6891280.7063190064771772Clontech SMART CDS Primer II A (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3498330.35855994386083617No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA2398160.24579845668341838Clontech SMART CDS Primer II A (100% over 26bp)
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC2062790.21142484173782755No Hit
GTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTAT1739810.17832113492109705No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT1713080.17558145418789No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG1583680.1623186525838126No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG1525720.1563780654047374Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA1488010.15251299393263723Clontech SMART CDS Primer II A (100% over 24bp)
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1464120.15006439787142078No Hit
GTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCC1345130.13786856508263273No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA1187410.12170311632687468No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG1185450.12150222690536007No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG1177860.120724292870005No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC1157560.11864365243288927No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG1109510.1137187867676967No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC1084410.1111461722370758No Hit
GGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTC1082720.11097295635832084No Hit
GGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG1079810.11067469706413331No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC1050840.10770542842062386No Hit
GCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTAT1045270.10713453348295222No Hit
GTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGC1025420.10510001561710262No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA1022820.10483352964978732No Hit
GCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTT1011110.10363331785084029No Hit
GAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCC996050.10208974913246775No Hit
GCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTG990380.10150860473451474No Hit
ATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAAC989740.10144300818871405No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT980760.10052260665544809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)