FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97854597
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC3235532433.06469495756035No Hit
TCGATACT2690228727.49210341135021No Hit
AGATCTGG2075599521.211057667530937No Hit
GTTGAGAA1666448017.02983866971523No Hit
CACCGCTA1861640.1902455333805115No Hit
AGATCTGA1823180.18631521215094268Illumina PCR Primer Index 9 (100% over 8bp)
TCGATACA1028680.10512331883600727No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)