FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47057212
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACAGGTAAGTAGTCCCTGATATA4198970.8923116822135573No Hit
TTAGGCAGTCTCTCTGGAACAACGCC3886100.8258245303610422No Hit
ATGGGAGAGGAACTGCCGTACAATAG3809650.8095783490105618No Hit
ATGGGAGAGGAACTGCTCTAGGTCGA3677070.7814041341845752No Hit
GGCTCGATCAGTGCATTCAGACGGAT3600460.7651239516697249No Hit
ATGGGAGAGGAACTGCATCAACATGC3510270.7459579203289817No Hit
TTAGGCAGTCTCTCTGAAATCATATA3441830.7314139222697681No Hit
CAGATCAGTAGAAGGATAGCGCTTAG3431340.7291847209307682No Hit
AACTTTCTCGGCGCTATTTTACGGTT3388660.7201149103351044No Hit
TATGCCCTCCAACCAACTCACTAACC3336800.709094282933719No Hit
AAACCTGCACAGGAGTTTAGCACGGT3250320.6907166535918022No Hit
AGATCTGGTGTTTGGTATGCAAACTG3162420.6720372639161029No Hit
AAGTCTGCAGCGTTCGACATCCTTGT3096020.6579267807026051No Hit
AGAATAGTCAGCTGGCTATCGCAGGC3050890.6483363272775277No Hit
AAGGCAGGTACGCACCGCTTAGGCGC3050700.6482959508948384No Hit
AACTCAGGTGATGTGGGCGACCTTCG3013700.6404331816343051No Hit
ACTATCTGTAAGGATTCGGGTGCTAC2979240.6331101808581435No Hit
GTGGGTCAGATCCCGCGATACGTTCC2970250.6311997404351112No Hit
AAACCTGCACAGGAGTAAACTCATCC2853760.6064447677010699No Hit
TGCTGCTAGATGAGAGAAAGCCTACG2823160.5999420450153315No Hit
GTGGGTCAGATCCCGCGCATTTGTGT2814070.598010353864568No Hit
TGCACCTCAATAAGCAGCCATTCACG2806070.5963102956460744No Hit
CACATTTAGCGTGAGTATAAGGCTCA2784970.5918263920947974No Hit
AAACCTGCACAGGAGTAGGATAGCGC2779490.5906618522151291No Hit
ACTATCTGTAAGGATTGACATTGCGC2774620.589626941774621No Hit
AGAATAGTCAGCTGGCAATGAGGCTA2774350.5895695648097469No Hit
AAACCTGCACAGGAGTCCGGCATGGC2753580.5851557886599826No Hit
AAGTCTGCAGCGTTCGGTCGTTGCGT2747680.5839019957238435No Hit
CACATTTAGCGTGAGTTCACTGCCCT2743060.5829202121026634No Hit
TTTGGTTGTGTCGCTGACGACGTGTC2727130.5795349711750879No Hit
TGCACCTCAATAAGCACTTGACTTGA2711270.5761646057569242No Hit
AAACCTGCACAGGAGTAAGCACAATC2701260.574037407911034No Hit
CTTGGCTGTATGCTTGCCCAACACTA2693450.5723777260752294No Hit
CAGATCAGTAGAAGGATGGTTAACAC2686500.570900800497913No Hit
GAACCTACAATCCGATAGTTGACTGA2682320.57001252007875No Hit
GTACTCCAGCCTATGTTCCTATCAGC2656280.5644788305775531No Hit
CAGCATATCCAGAGGATCGGAGAATA2647120.5625322639173779No Hit
TGCTGCTAGATGAGAGTTCGATTAGG2642880.5616312330615763No Hit
CTCTGGTTCTCCGGTTAACATCAGCA2631700.5592554017012313No Hit
GAGCAGACATGCAATCCAAACACCCT2631630.5592405261918194No Hit
ACGGGTCCATTTGCTTTATCACTCGG2614020.5554982730383602No Hit
GTGGGTCAGATCCCGCATTGCCATCT2605180.5536197087069247No Hit
GTGGGTCAGATCCCGCTGGTTCCACA2603030.5531628180607046No Hit
CACAGTAGTATAGTAGTCCCCATCAA2594370.551322505039185No Hit
TGCTACCCATGGTAGGCTATTTTTGG2592720.5509718680316207No Hit
CACATTTAGCGTGAGTCGAATATGTC2573650.5469193542532864No Hit
TGCACCTCAATAAGCATTCGACTCAT2570000.5461437026910987No Hit
GTGGGTCAGATCCCGCACTGGTTGAA2550690.5420401871662095No Hit
GTACTCCAGCCTATGTTGAGTTTTCC2539790.5397238578435118No Hit
TGCTACCCATGGTAGGTATAGACTCT2525580.5367041294329123No Hit
CTTGGCTGTATGCTTGGAATGCACGG2522250.5359964801994644No Hit
ACGGGTCCATTTGCTTCGAATAAGGG2513000.5340307878843311No Hit
AAACCTGCACAGGAGTTGATACCTCC2476180.5262062699337139No Hit
TGCTGCTAGATGAGAGCAGCCCACAA2463020.5234096741642917No Hit
AAACCTGCACAGGAGTTTTTAGTCGA2459370.522634022602104No Hit
AGAATAGTCAGCTGGCAGGCTTGTGT2438620.518224496597886No Hit
GTGGGTCAGATCCCGCCGTTGTACAC2432470.5169175768424189No Hit
TGCTGCTAGATGAGAGGACACGCATG2420620.5143993656062752No Hit
CTTGGCTGTATGCTTGTTAAGACGGA2419420.5141443568735011No Hit
AGGCCACTCTGCGTAAGGTTGGCTCA2417540.5137448431921551No Hit
AAACCTGCACAGGAGTATGATGGGCA2410780.5123082939975279No Hit
AAGTCTGCAGCGTTCGCTAACCCCAA2409840.5121085371568549No Hit
CGGAGCTCACGACTCGTGGCGCCACG2381180.5060180785891013No Hit
ATGGGAGAGGAACTGCAGTAAGGAGC2370830.5038186282689251No Hit
GGCTCGATCAGTGCATCATAAAACAG2345150.4983614413875603No Hit
GTGGGTCAGATCCCGCGGACCCTGGA2343990.4981149329458787No Hit
GGACAGAAGCAGCCTCTTAACCCCAG2343190.4979449271240294No Hit
TGCCCATAGCCCAGCTCTATCGCCGT2319620.4929361305977923No Hit
ATCATCTCAATCTGCACCCGTCGACA2318170.4926279950456904No Hit
AGAATAGTCAGCTGGCGGCGGGTCTC2315000.4919543469766122No Hit
AGAATAGTCAGCTGGCTTTGCGGCCT2310550.49100868959257515No Hit
AGAATAGTCAGCTGGCGAGGACGCAG2307510.49036266746954754No Hit
GCTCCTATCCGTACAAATCAGAACAA2288580.4863399047100368No Hit
AGAATAGTCAGCTGGCGTGTCTATCA2283370.4852327417952428No Hit
CACATTTAGCGTGAGTTTAATCTGAA2282930.4851392385932256No Hit
GGCTCGATCAGTGCATGTGCGGAGCC2276680.4838110681100274No Hit
GTGGGTCAGATCCCGCACCTACATAA2269470.48227889064061No Hit
AAGTCTGCAGCGTTCGGTCTCTGCTC2249930.47812649844193916No Hit
TTTGGTTGTGTCGCTGACCCTATCGT2243190.4766941993928582No Hit
ACGGGTCCATTTGCTTGCGTCCGTGT2230920.47408673510024346No Hit
AAGTCTGGTAGCCTCGACCCGTTTTC2229840.4738572272407469No Hit
GAAACTCCAGTATGCTAAACGTCTGC2214780.47065686764443243No Hit
TGCCCATAGCCCAGCTAATCCAGCGT2210110.4696644586593868No Hit
GGCTCGATCAGTGCATGCTTCAATGT2201070.46774339287248895No Hit
AAGGCAGGTACGCACCTAGACTACGC2199690.4674501328297988No Hit
TCGTACCAGCTGCAAGCCCGCAGAGC2193950.4662303410580295No Hit
AAGTCTGCAGCGTTCGTAGACGTGGG2192420.4659052049237426No Hit
GAAACTCCAGTATGCTTCGGTTAACG2192420.4659052049237426No Hit
TGCTGCTAGATGAGAGAATCCTCCGA2190770.4655545679161783No Hit
ACGGGTCCATTTGCTTCAGACTTCCG2162260.4594959854400214No Hit
AGAGCGATCAAGAAGTCGATAGGGCT2151980.457311410629257No Hit
CAGATCAGTAGAAGGAGACTTGCAGC2145580.455951364054462No Hit
ATGGGAGAGGAACTGCCGACTATACC2137170.45416417785227053No Hit
ATCATCTCAATCTGCATTCGGGTCCC2135720.4538560423001685No Hit
ACGGGTCCATTTGCTTTACACAGTAA2135130.4537306630065546No Hit
CAGATCAGTAGAAGGAGTCTCAATCC2134310.45355640703915906No Hit
TGCTACCCATGGTAGGTCTCGTGCCT2123990.4513633319373022No Hit
CGGAGCTCACGACTCGTACTGTCTAT2112180.44885362099225085No Hit
ATGGGAGAGGAACTGCGCGTACAGCT2106080.44755732660064945No Hit
AAACCTGCACAGGAGTTAAAACTGCG2101560.44659679370720057No Hit
GTGGGTCAGATCCCGCCCAATACTCA2092750.44472460459408436No Hit
TGCTACCCATGGTAGGTGATCTTGAG2092040.444573724427193No Hit
CGGTTAACATGCATTACAAGTGTTCT2077270.441434991941299No Hit
AACTCAGGTGATGTGGTACCCGTCAG2076150.4411969837907099No Hit
CGGAGCTCACGACTCGTAGATTGCTC2059080.437569484566999No Hit
CAGATCAGTAGAAGGACCGCCCTTAT2057440.4372209726322078No Hit
ATCCGAAAGACGCACACGGCAGCGTG2051200.43589492722178275No Hit
GCCTCTAGTCGCGGTTCGCCCTGGCT2048840.43539341004732707No Hit
CAGCATATCCAGAGGAGCTAACCTAC2030890.43157890441958185No Hit
AAACCTGCACAGGAGTAGGTCTTCCC2027320.430820253439579No Hit
AAGTCTGCAGCGTTCGTCTGGGTCTT2017430.4287185564669662No Hit
CGCGGTACAAAGGAAGAGTCGTTCGC1982100.42121067435954346No Hit
CACAGTAGTATAGTAGCTAGGAAGGT1981220.4210236679555091No Hit
TCGTACCAGCTGCAAGATTGCCCTGA1979700.42070065689399533No Hit
TGGTTCCGTCCAAGTTGTCAGCGTTG1966710.41794018736171623No Hit
AGTCTTTCAAACTGTCCTCAACCCTC1962610.4170689075247382No Hit
CACATTTAGCGTGAGTTCCTTGTCTT1960880.4167012699349889No Hit
AACTCAGGTGATGTGGACAACGGGAT1929180.40996478924420765No Hit
GGTGAAGCAATGGAGCTATCTTAAGT1907460.4053491311809973No Hit
CCCATACTCCAAAGTCATGTAGAGTG1901180.40401458547947977No Hit
ATCCGAAAGACGCACAGCCTGAGTGG1900080.40378082747443683No Hit
TGTTCCGCAATAAGCAGGTTTCCCAA1893590.40240165524468385No Hit
GGCTCGATCAGTGCATAAATGTTTGC1890310.4017046313751015No Hit
GTGGGTCAGATCCCGCGCCCTATCTG1869370.3972547289881942No Hit
ATGGGAGAGGAACTGCCTCTCACATC1851680.39349547525255No Hit
CTTGGCTGTATGCTTGTATGCGCGGG1845000.39207592664010776No Hit
AGAATAGTCAGCTGGCTGTACAACGC1839910.3909942645985912No Hit
AACTTTCTCGGCGCTACTTGTGGCAA1825040.387834281384966No Hit
GGCTCGATCAGTGCATCCTGTAGTCT1821480.3870777554777364No Hit
AAGGCAGGTACGCACCAAAATCCGAG1813990.38548607597067164No Hit
ATAACGCGTTCCACGGTCCATTAGTC1810160.38467217309856777No Hit
TGGTTCCGTCCAAGTTGTATTGACGG1793970.38123168027889115No Hit
ACGAGGACACACTGCGAAGAGGCCTT1772430.3766542735255969No Hit
ACTATCTGTAAGGATTTTCAGCCCTG1764340.37493508965214517No Hit
GCTTCCAGTGCGAAACCGCGTATCAA1756540.3732775328891138No Hit
GTACTCCAGCCTATGTAATTATGAAC1747540.3713649673933084No Hit
CGGAGCTCACGACTCGGCCATTCCCC1741860.3701579260581779No Hit
CTCGAGGGTAACGCGATGGGATGAGG1739700.36969891033918456No Hit
CACAGTAGTATAGTAGTCTAGTCAAA1732280.36812210634153164No Hit
AAACCTGCACAGGAGTCAACTCTCCG1720190.36555289335883306No Hit
GGCTCGATCAGTGCATCTCGTAGCGG1703720.3620528985015092No Hit
GTGGGTCAGATCCCGCAAAGGCGGTA1696530.360524971177638No Hit
AGAATAGTCAGCTGGCCAGAGGCTCG1688820.35888654006956466No Hit
TTGACTTGTCATTAGCAAGGGTTATT1659750.3527089535181132No Hit
CGGTTAACATGCATGTGGGCGGATGC1653400.35135953230718386No Hit
ACTATCTGTAAGGATTCATATGCATA1652950.35126390403239355No Hit
CACATTTAGCGTGAGTGTAAGACGTG1648650.35035012273995325No Hit
AGAATAGTCAGCTGGCAATGCTTCAT1618810.34400890558497177No Hit
TTTATGCCAGCTGCACTGAAAATGCG1614830.34316312662127113No Hit
AGAATAGTCAGCTGGCCCTGAGTCCA1611860.3425319800076554No Hit
AAACCTGCACAGGAGTCGCGGTCTAT1573170.33431007344846525No Hit
TTTATGCCAGCTGCACCGCGCGCTCT1568150.33324328691636046No Hit
AAGTCTGCAGCGTTCGAGCATACGCG1528750.324870500190279No Hit
TTTGGTTGTGTCGCTGTGGGTTCTAC1518820.3227603029265737No Hit
GTAGGCCCAGCAGTTTGATCATATGG1506180.3200742109413537No Hit
ATCTACTTCCCACTTGACGCCCCTAG1504760.3197724506075711No Hit
TCGTACCAGCTGCAAGATTAGTAAGT1435420.305037195998777No Hit
CTAAGACGTTAAGTAGGGCCACAAAG1376350.29248439112797414No Hit
TGGTTCCGTCCAAGTTCTTGTGGTCA1275210.27099140510066766No Hit
ATCCGAAAGACGCACACCCATCATCG1263070.2684115667541035No Hit
ATCTACTTCCCACTTGAGGGATCAGC1242250.26398716524047366No Hit
AGAATAGTCAGCTGGCGATAAATGCG1240930.2637066556344222No Hit
AAGTCTGCAGCGTTCGGAGAGGGTAA1235780.26261224315626686No Hit
GTACTCCAGCCTATGTACTATTTAGG1192400.25339367746648483No Hit
CCTTCCCCAGGACCCTACTAGACCCA1188630.25259252503101964No Hit
CCTTCCCCAGGACCCTGATTGCGACT1169240.24847200892394558No Hit
ACTATCTGTAAGGATTAACGCAAACT1168320.24827650222881884No Hit
CCTTCCCCAGGACCCTGGCAACGCGC1151800.24476588200762933No Hit
CTCGAGGGTAACGCGAAGCCGTGGGT1103850.23457615806053278No Hit
TAGTGGTGTGCTGTATACAAGTCATA1102730.23433814990994364No Hit
ACTATCTGTAAGGATTAAGCACGTAG1097210.233165109739183No Hit
GTACTCCAGCCTATGTGACTCGTCGA1076390.22874070822555317No Hit
GTGGGTCAGATCCCGCGTCTGTTACT1064640.22624374771714056No Hit
TCGTACCAGCTGCAAGATAGTTCCCG1063970.22610136784134172No Hit
CCGTGGACAGATCGGAGGGCAATACC1049440.22301363710200256No Hit
ACGGGTCCATTTGCTTTGCCTATACT991710.21074559198279744No Hit
CTTGGCTGTATGCTTGCACTGTTCAG986010.20953430050212069No Hit
GGTGAAGTCTTAGCATGTACTGCTCG948510.20156527760293153No Hit
AGGCCACTCTGCGTAACGGATAACTT946950.2012337662503252No Hit
AGAATAGTCAGCTGGCGCACCATGCG884920.18805193983867977No Hit
GAAACTCCAGTATGCTAGGTCACTTA853450.1813643358216802No Hit
TGGTTCCGTCCAAGTTAACTCTGAGT843840.17932214088671466No Hit
CCTTCCCCAGGACCCTCAATTGCCTT812690.17270253919845485No Hit
GTACTCCAGCCTATGTATCAATCGTT795960.16914729244902993No Hit
ACGTCAATCGCAAACTCGACAACACG750040.15938895827487612No Hit
TGCTACCCATGGTAGGCTTCATCCAC702290.14924173578324187No Hit
AGAATAGTCAGCTGGCTTTTCAAGTT654530.13909238821883455No Hit
ACACCGGGTAGGAGTCCCCGTTGACT652930.13875237657513584No Hit
ATAACGCGTTCCACGGGACGCTCGCT533840.11344488492008409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)